Knight, Rob ![]() ![]() ![]() ![]() ![]() ![]() |
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Abstract
Scar formation during wound repair can be devastating for affected individuals. Our group previously documented the therapeutic potential of novel progenitor cell populations from the non-scarring buccal mucosa. These Oral Mucosa Lamina Propria-Progenitor Cells (OMLP-PCs) are multipotent, immunosuppressive, and antibacterial. Small extracellular vesicles (sEVs) may play important roles in stem cell–mediated repair in varied settings; hence, we investigated sEVs from this source for wound repair. We created an hTERT immortalized OMLP-PC line (OMLPPCL ) and confrmed retention of morphology, lineage plasticity, surface markers, and functional properties. sEVs isolated from OMLP-PCL were analyzed by nanoparticle tracking analysis, Cryo-EM and fow cytometry. Compared to bone marrow–derived mesenchymal stromal cells (BM-MSC) sEVs, OMLP-PCL sEVs were more potent at driving wound healing functions, including cell proliferation and wound repopulation and downregulated myofbroblast formation. A reduced scarring potential was further demonstrated in a preclinical in vivo model. Manipulation of OMLP-PCL sEVs may provide novel options for non-scarring wound healing in clinical settings.
Item Type: | Article |
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Date Type: | Publication |
Status: | Published |
Schools: | Dentistry Medicine |
Publisher: | Oxford University Press |
ISSN: | 2157-6564 |
Funders: | Dunhill Medical Trust |
Date of First Compliant Deposit: | 29 June 2022 |
Date of Acceptance: | 28 April 2022 |
Last Modified: | 05 Jan 2024 03:11 |
URI: | https://orca.cardiff.ac.uk/id/eprint/150847 |
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