Anti-fibrotic potential of Tomentosenol A, a constituent of cerumen from the Australian native stingless bee, Tetragonula carbonaria

Hamilton, Karina D., Czajkowski, Daniel, Kong, Nicolas J., Tran, Trong D., Gustafson, Kirk R., Pauly, Gary, Boyle, Glen M., Simmons, Jacinta L., Steadman, Robert ORCID: https://orcid.org/0000-0002-1303-2496, Moseley, Ryan ORCID: https://orcid.org/0000-0002-2812-6735, Brooks, Peter R., Ogbourne, Steven M. and Russell, Fraser D. 2022. Anti-fibrotic potential of Tomentosenol A, a constituent of cerumen from the Australian native stingless bee, Tetragonula carbonaria. Antioxidants 11 (8) , e1604. 10.3390/antiox11081604

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Abstract

Bioactivity-guided fractionation was used to isolate two compounds, tomentosenol A (1) and torellianone A (2), from a cerumen extract from Tetragonula carbonaria. The anti-fibrotic activity of these compounds was examined using human cultured neonatal foreskin fibroblasts (NFF) and immortalised keratinocytes (HaCaTs). Tomentosenol A (1), inhibited NFF and HaCaT cell proliferation and prevented NFF and HaCaT scratch wound repopulation at 12.5−25 µM concentrations. These inhibitory effects were associated with reduced cell viability, determined by tetrazolium dye (MTT) and sulforhodamine B (SRB) assays. Compound 1 further inhibited transforming growth factor-β1 (TGF-β1)-stimulated, NFF-myofibroblast differentiation and soluble collagen production; and was an effective scavenger of the model oxidant, 2,2-diphenyl-1-picrylhydrazyl (DPPH·), with an EC50 value of 44.7 ± 3.1 µM. These findings reveal significant anti-fibrotic potential for cerumen-derived tomentosenol A (1).

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Dentistry Medicine Cardiff Institute Tissue Engineering Repair (CITER)
Additional Information:	License information from Publisher: LICENSE 1: URL: https://creativecommons.org/licenses/by/4.0/
Publisher:	MDPI
Date of First Compliant Deposit:	22 August 2022
Date of Acceptance:	15 August 2022
Last Modified:	23 May 2023 17:14
URI:	https://orca.cardiff.ac.uk/id/eprint/152097

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