PAX6-positive microglia evolve locally in hiPSC-derived ocular organoids

Shiraki, Nobuhiko, Maruyama, Kazuichi, Hayashi, Ryuhei, Oguchi, Akiko, Murakawa, Yasuhiro, Katayama, Tomohiko, Takigawa, Toru, Sakimoto, Susumu, Quantock, Andrew J. ORCID: https://orcid.org/0000-0002-2484-3120, Tsujikawa, Motokazu and Nishida, Kohji 2022. PAX6-positive microglia evolve locally in hiPSC-derived ocular organoids. Stem Cell Reports 17 (2) , pp. 221-230. 10.1016/j.stemcr.2021.12.009

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Abstract

Microglia are the resident immune cells of the central nervous system (CNS). They govern the immunogenicity of the retina, which is considered to be part of the CNS; however, it is not known how microglia develop in the eye. Here, we studied human-induced pluripotent stem cells (hiPSCs) that had been expanded into a self-formed ectodermal autonomous multi-zone (SEAM) of cells that partially mimics human eye development. Our results indicated that microglia-like cells, which have characteristics of yolk-sac-like linage cells, naturally develop in 2D eye-like SEAM organoids, which lack any vascular components. These cells are unique in that they are paired box protein 6 (PAX6)-positive, yet they possess some characteristics of mesoderm. Collectively, the data support the notion of the existence of an isolated, locally developing immune system in the eye, which is independent of the body’s vasculature and general immune system.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Optometry and Vision Sciences
Publisher:	Cell Press
ISSN:	2213-6711
Date of First Compliant Deposit:	21 February 2023
Date of Acceptance:	14 December 2021
Last Modified:	03 May 2023 07:48
URI:	https://orca.cardiff.ac.uk/id/eprint/157166

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