Isolation and characterization of tissue inhibitors of metalloproteinases (TIMP-1 and TIMP-2) from human rheumatoid synovial fluid

Osthues, Anja, Knauper, Vera ORCID: https://orcid.org/0000-0002-3965-9924, Oberhoff, Renate, Reinke, Heinz and Tschesche, Harald 1992. Isolation and characterization of tissue inhibitors of metalloproteinases (TIMP-1 and TIMP-2) from human rheumatoid synovial fluid. FEBS Letters 296 (1) , pp. 16-20. 10.1016/0014-5793(92)80393-U

Full text not available from this repository.

Abstract

The tissue inhibitors of metalloproteinases TIMP-1 and TIMP-2 were purified to apparent homogeneity from human rheumatoid synovial fluid (HRSF). The inhibitors were isolated by dissociation of non-covalent gelatinase/TIMP complexes. TIMP-1 migrated as a single polypeptide with M r 28 500 on SDS-PAGE, while the M r of TIMP-2 was 21 000. The inhibitory activity was stable under heat and acid pH. N-terminal sequence data were obtained for the first 15 residues of both inhibitors and showed identity to the human fibroblast inhibitors TIMP-1 and TIMP-2. This is the first demonstration that TIMP-1 and TIMP-2 can be directly purified from human rheumatoid synovial fluid. The complex formation between the metalloproteinase inhibitors and leucocyte metalloproteinases was shown by immunoblotting.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry
Publisher:	Federation of European Biochemical Societies
ISSN:	1873-3468
Last Modified:	31 Aug 2023 15:15
URI:	https://orca.cardiff.ac.uk/id/eprint/161629

Actions (repository staff only)

Edit Item