Comparative hydrodynamic and nanoscale imaging study on the interactions of teicoplanin-A2 and bovine submaxillary mucin as a model ocular mucin

Chun, Taewoo, Pattem, Jacob ORCID: https://orcid.org/0000-0003-0276-7996, Gillis, Richard B., Dinu, Vlad T., Yakubov, Gleb E., Corfeld, Anthony P. and Harding, Stephen E. 2023. Comparative hydrodynamic and nanoscale imaging study on the interactions of teicoplanin-A2 and bovine submaxillary mucin as a model ocular mucin. Scientific Reports 13 , 11367. 10.1038/s41598-023-38036-6

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Abstract

Glycopeptide antibiotics are regularly used in ophthalmology to treat infections of Gram-positive bacteria. Aggregative interactions of antibiotics with mucins however can lead to long exposure and increases the risk of resistant species. This study focuses on the evaluation of potential interactions of the last line of defence glycopeptide antibiotic teicoplanin with an ocular mucin model using precision matrix free hydrodynamic and microscopic techniques: sedimentation velocity in the analytical ultracentrifuge (SV-AUC), dynamic light scattering (DLS) and atomic force microscopy (AFM). For the mixtures of teicoplanin at higher doses (1.25 mg/mL and 12.5 mg/mL), it was shown to interact and aggregate with bovine submaxillary mucin (BSM) in the distributions of both sedimentation coefficients by SV-AUC and hydrodynamic radii by DLS. The presence of aggregates was confirmed by AFM for higher concentrations. We suggest that teicoplanin eye drop formulations should be delivered at concentrations of < 1.25 mg/mL to avoid potentially harmful aggregations.

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Dentistry
Publisher:	Nature Research
ISSN:	2045-2322
Date of First Compliant Deposit:	15 September 2023
Date of Acceptance:	1 July 2023
Last Modified:	16 Sep 2023 01:54
URI:	https://orca.cardiff.ac.uk/id/eprint/162533

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