A multi-scale biophysical approach to develop structure-property relationships in Oral Biofilms

Pattem, J. ORCID: https://orcid.org/0000-0003-0276-7996, Davrandi, M., Aguayo, S., Allen, E., Spratt, D. and Bozec, L. 2018. A multi-scale biophysical approach to develop structure-property relationships in Oral Biofilms. Scientific Reports 8 (1) , 5691. 10.1038/s41598-018-23798-1

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Abstract

Over the last 5-10 years, optical coherence tomography (OCT) and atomic force microscopy (AFM) have been individually applied to monitor the morphological and mechanical properties of various single-species biofilms respectively. This investigation looked to combine OCT and AFM as a multi-scale approach to understand the role sucrose concentration and age play in the morphological and mechanical properties of oral, microcosm biofilms, in-vitro. Biofilms with low (0.1% w/v) and high (5% w/v) sucrose concentrations were grown on hydroxyapatite (HAP) discs from pooled human saliva and incubated for 3 and 5 days. Distinct mesoscale features of biofilms such as regions of low and high extracellular polymeric substances (EPS) were identified through observations made by OCT. Mechanical analysis revealed increasing sucrose concentration decreased Young's modulus and increased cantilever adhesion (p < 0.0001), relative to the biofilm. Increasing age was found to decrease adhesion only (p < 0.0001). This was due to mechanical interactions between the indenter and the biofilm increasing as a function of increased EPS content, due to increasing sucrose. An expected decrease in EPS cantilever contact decreased adhesion due to bacteria proliferation with biofilm age. The application OCT and AFM revealed new structure-property relationships in oral biofilms, unattainable if the techniques were used independently. © 2018 The Author(s).

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry
Publisher:	Nature Research
Date of First Compliant Deposit:	13 November 2023
Last Modified:	13 Nov 2023 15:39
URI:	https://orca.cardiff.ac.uk/id/eprint/162537

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