The aorta-gonad-mesonephros-derived stroma cell line DAS104-4 induces differentiation of leukemic cells

Pramanik, Kallal, Trüpschuch, Sandra, Greiner, Axel, Ruthardt, Martin ORCID: https://orcid.org/0000-0003-1021-3811, Henschler, Reinhard and Müller, Albrecht M. 2005. The aorta-gonad-mesonephros-derived stroma cell line DAS104-4 induces differentiation of leukemic cells. Leukemia Research 32 (5) , pp. 781-789. 10.1016/j.leukres.2007.09.014

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Abstract

While critical steps in the regulation of leukemia cell development have been intensively studied in recent years, less is known about the interactions of leukemic cells with their stroma. Previously, we have shown that human acute myeloid leukemia (AML) cells differentiate upon injection into murine blastocysts. We here describe that human AML Kasumi-1 cells, cocultured with murine aorta-gonad-mesonephros (AGM) region-derived DAS104-4 stromal cells, decrease proliferation and colony formation efficiency; and up-regulate myelo-monocytic cell surface markers. Gene expression analysis showed decreased transcription of the AML1-ETO fusion gene and increased transcription of p16 (INK4A), p21 (WAF1) and C/EBPalpha genes. Coculture can induce myeloid differentiation also in patient-derived AML cells. Our findings strengthen the notion that the embryonic milieu can regulate the proliferation and differentiation of leukemic cells.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Publisher:	Elsevier
ISSN:	0145-2126
Date of Acceptance:	16 September 2007
Last Modified:	22 Feb 2024 16:00
URI:	https://orca.cardiff.ac.uk/id/eprint/166075

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