Untargeted metabolomics reveals metabolic state of Bifidobacterium bifidum in the biofilm and planktonic states

Sadiq, Faizan Ahmed ORCID: https://orcid.org/0000-0003-1596-4155, Yan, Bowen, Zhao, Jianxin, Zhang, Hao and Chen, Wei 2020. Untargeted metabolomics reveals metabolic state of Bifidobacterium bifidum in the biofilm and planktonic states. LWT - Food Science and Technology 118 , 108772. 10.1016/j.lwt.2019.108772

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Abstract

Bifidobacterium bifidum is the major and dominant coloniser of the human gut which can form biofilms on mucosa, epithelial cells, and food residues in the gut lumen. This study provided metabolic insights into B. bifidum biofilm and planktonic states using untargeted metabolomics. The two states were clearly distinguishable by PCA and PLS-DA. Out of the total 173 metabolites, only 48 metabolites showed fold change (FC) ≥ 1 (p < 0.05) in the planktonic state compared with biofilms, and similarly only 16 metabolites expressed with FC ≥ 1 (p < 0.05) in biofilms as compared to planktonic cells. Aminoacyl-tRNA biosynthesis, alanine, aspartate and glutamate metabolism, nitrogen metabolism, citrate cycle (TCA), and arginine and proline metabolism were among the five most affected metabolic pathways that were down regulated in biofilms compared with planktonic cells. Among amino acids, l-histidine, l-alanine, and d-methionine were significantly more expressed. Metabolites involved in nucleotides (adenine, guanine, thymine, and uracil) synthesis/regulation were prevalent in biofilms. Similarly, Poly-N-acetylglucosamine, an exopolysaccharide which serves as a major component of biofilm matrix, was more expressed in biofilms (FC ≥ 4). These comprehensive insights emphasize the fact that functional claims related to metabolism of this probiotic species should be state-dependent.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry
Publisher:	Elsevier
ISSN:	0023-6438
Last Modified:	03 May 2024 01:29
URI:	https://orca.cardiff.ac.uk/id/eprint/168415

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