Immunoregulatory effect of Lactobacillus paracasei VL8 exopolysaccharide on RAW264.7 cells by NF-κB and MAPK pathways

Gao, Jie, Li, Qi, Liu, Yuwei, Yang, Bing, Sadiq, Faizan Ahmed ORCID: https://orcid.org/0000-0003-1596-4155, Li, Xiyu, Mi, Si and Sang, Yaxin 2022. Immunoregulatory effect of Lactobacillus paracasei VL8 exopolysaccharide on RAW264.7 cells by NF-κB and MAPK pathways. Journal of Functional Foods 95 , 105166. 10.1016/j.jff.2022.105166

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Abstract

This study explored the immunoregulatory mechanism of VL8-EPS, obtained from Viili. An RAW264.7 macrophage cell-line was used along with real-time quantitative PCR to evaluate the impact of VL8-EPS on mRNA expression of IL-1β, TNF-α, IL-6, IL-10, and TLR4. Moreover, western blot assay was conducted to study the signal pathway of NF-κB and MAPK. The VL8-EPS was found to upregulate the mRNA expression of these cytokines and TLR4, while it promoted protein phosphorylation of p65, IκBα, ERK1/2, and JNK. The immunofluorescence staining and inhibitor blocking experiments proved that TLR4, NF-κB, and MAPK signal pathway were involved to secrete immune-enhancing substances. In conclusion, VL8-EPS was involved to activate downstream signal pathways of NF-κB and MAPK through TLR4 identification, which caused an increase in mRNA expression and cytokines release, ultimately exerting immune regulation.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry
Publisher:	Elsevier
ISSN:	1756-4646
Last Modified:	07 May 2024 12:00
URI:	https://orca.cardiff.ac.uk/id/eprint/168594

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