Ashraf, Sanju, Tay, Ye Dee, Kelly, David A. and Sawin, Kenneth E.
2021.
Microtubule-independent movement of the fission yeast nucleus.
Journal of Cell Science
134
(6)
, jcs253021.
10.1242/jcs.253021
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Abstract
Movement of the cell nucleus typically involves the cytoskeleton and either polymerization-based pushing forces or motor-based pulling forces. In the fission yeast Schizosaccharomyces pombe, nuclear movement and positioning are thought to depend on microtubule polymerization-based pushing forces. Here, we describe a novel, microtubule-independent, form of nuclear movement in fission yeast. Microtubule-independent nuclear movement is directed towards growing cell tips, and it is strongest when the nucleus is close to a growing cell tip, and weakest when the nucleus is far from that tip. Microtubule-independent nuclear movement requires actin cables but does not depend on actin polymerization-based pushing or myosin V-based pulling forces. The vesicle-associated membrane protein (VAMP)-associated proteins (VAPs) Scs2 and Scs22, which are critical for endoplasmic reticulum–plasma membrane contact sites in fission yeast, are also required for microtubule-independent nuclear movement. We also find that in cells in which microtubule-based pushing forces are present, disruption of actin cables leads to increased fluctuations in interphase nuclear positioning and subsequent altered septation. Our results suggest two non-exclusive mechanisms for microtubule-independent nuclear movement, which may help illuminate aspects of nuclear positioning in other cells.
Item Type: | Article |
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Date Type: | Published Online |
Status: | Published |
Schools: | Schools > Biosciences |
Publisher: | The Company of Biologists |
ISSN: | 0021-9533 |
Funders: | wellcome Trust |
Date of First Compliant Deposit: | 30 July 2024 |
Date of Acceptance: | 9 February 2021 |
Last Modified: | 30 Jul 2024 10:45 |
URI: | https://orca.cardiff.ac.uk/id/eprint/170124 |
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