Microtubule-independent movement of the fission yeast nucleus

Ashraf, Sanju, Tay, Ye Dee, Kelly, David A. and Sawin, Kenneth E. 2021. Microtubule-independent movement of the fission yeast nucleus. Journal of Cell Science 134 (6) , jcs253021. 10.1242/jcs.253021

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Abstract

Movement of the cell nucleus typically involves the cytoskeleton and either polymerization-based pushing forces or motor-based pulling forces. In the fission yeast Schizosaccharomyces pombe, nuclear movement and positioning are thought to depend on microtubule polymerization-based pushing forces. Here, we describe a novel, microtubule-independent, form of nuclear movement in fission yeast. Microtubule-independent nuclear movement is directed towards growing cell tips, and it is strongest when the nucleus is close to a growing cell tip, and weakest when the nucleus is far from that tip. Microtubule-independent nuclear movement requires actin cables but does not depend on actin polymerization-based pushing or myosin V-based pulling forces. The vesicle-associated membrane protein (VAMP)-associated proteins (VAPs) Scs2 and Scs22, which are critical for endoplasmic reticulum–plasma membrane contact sites in fission yeast, are also required for microtubule-independent nuclear movement. We also find that in cells in which microtubule-based pushing forces are present, disruption of actin cables leads to increased fluctuations in interphase nuclear positioning and subsequent altered septation. Our results suggest two non-exclusive mechanisms for microtubule-independent nuclear movement, which may help illuminate aspects of nuclear positioning in other cells.

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Biosciences
Publisher:	The Company of Biologists
ISSN:	0021-9533
Funders:	wellcome Trust
Date of First Compliant Deposit:	30 July 2024
Date of Acceptance:	9 February 2021
Last Modified:	30 Jul 2024 10:45
URI:	https://orca.cardiff.ac.uk/id/eprint/170124

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