Dinitrophenyl (DNP) hapten sandwich staining (DHSS) procedure: A 10 year review of its principle reagents and applications

Jasani, Bharat, Thomas, N. D., Navabi, H., Millar, David M., Newman, G. R., Gee, Julia Margaret Wendy ORCID: https://orcid.org/0000-0001-6483-2015 and Williams, E. D. 1992. Dinitrophenyl (DNP) hapten sandwich staining (DHSS) procedure: A 10 year review of its principle reagents and applications. Journal of Immunological Methods 150 (1-2) , pp. 193-198. 10.1016/0022-1759(92)90078-8

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Abstract

Over the past 10 years an immunoperoxidase method using dinitrophenyl (DNP) hapten-labelled primary or secondary probes has been devised. Its widely successful application in research and diagnostic work has depended upon the development of certain key reagents. These include a novel non-deleterious DNP labelling compound, a unique multivalent monoclonal bridge antibody, and an efficient DNP hapten substituted or anti-DNP linked marker enzyme. In this article the development of these reagents and various modifications of the basic technique are reviewed in conjunction with the special applications accruing from their use.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Pharmacy Cardiff Catalysis Institute (CCI)
Subjects:	R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) R Medicine > RM Therapeutics. Pharmacology
Uncontrolled Keywords:	Dinitrophenyl; Immunoperoxidase; Dinitrophenyl-hapten procedure; Dinitrophenyl localization system; Immunolocalization; Immunoassay
Publisher:	Elsevier
ISSN:	0022-1759
Last Modified:	06 May 2023 01:14
URI:	https://orca.cardiff.ac.uk/id/eprint/17089

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