Comparison of the effects of three sourdough postbiotics on high-fat diet-induced intestinal damage

Yu, Yujuan, Zhou, Min, Sadiq, Faizan Ahmed ORCID: https://orcid.org/0000-0003-1596-4155, Hu, Pengli, Gao, Feng, Wang, Juanxia, Liu, Aowen, Liu, Yue, Wu, Haili and Zhang, Guohua 2024. Comparison of the effects of three sourdough postbiotics on high-fat diet-induced intestinal damage. Food and Function 15 (18) , pp. 9053-9069. 10.1039/D4FO02948H Item availability restricted.

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Abstract

There is significant interest in using postbiotics as an intervention strategy to address obesity. This study assesses the efficacy of postbiotics derived from different sourdough strains (Lactiplantibacillus plantarum LP1, LP25, and Pediococcus pentosaceus PP18) in mitigating intestinal injury in zebrafish fed on a high-fat diet. We screened postbiotics for their anti-colon cancer cell effects and compared various preparation methods applied to live bacterial strains, including heat-killing at different temperatures, pH adjustments, and ultraviolet radiation exposure. Heat-killing at 120 °C proved to be the most effective preparation method. A marked variation in health effects was observed in the heat-killed microbial cells, as evidenced by their hydrophobicity and self-aggregation ability. A five-week high-fat dietary intervention study in zebrafish demonstrated that diets supplemented with 108 CFU g−1 K-LP25 significantly attenuated weight gain and body fat, along with reductions in FASN, Leptin, and SREBF1 mRNA expression. However, diets supplemented with 107 CFU g−1 K-PP18 only reduced Leptin and SREBF1 mRNA expression. K-PP18 was more effective at mitigating gut barrier damage, promoting colonic Occludin, ZO-1, and Claudin-1 levels. Additionally, K-LP25 supplementation markedly downregulated the pro-inflammatory cytokines TNF-α, IL-6, and IL-1β, reducing intestinal inflammation. Supplementation with K-LP1 and K-PP18 increased the abundance of Acinetobacter spp., whereas K-LP25 increased the abundance of Cetobacterium and Plesiomonas. Collectively, these findings suggest that inactivated strains confer protective effects against high-fat diet-induced intestinal damage in zebrafish, with variation observed across different species. Studying the effects of sourdough-derived postbiotics on gut health may open new avenues for dietary interventions to manage gut-related diseases.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry
Publisher:	Royal Society of Chemistry
ISSN:	2042-6496
Funders:	National Natural Science Foundation of China
Date of First Compliant Deposit:	22 August 2024
Date of Acceptance:	31 July 2024
Last Modified:	08 Oct 2024 11:47
URI:	https://orca.cardiff.ac.uk/id/eprint/171526

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