Integrated network pharmacology and transcriptomic approach reveal the role of equol in reducing colorectal cancer via regulating multiple cell cycle genes in HCT116 cells.

Mao, Kemin, Wang, Xianghong, Hou, Yakun, He, Xiaowei, Geng, Shuo, Sadiq, Faizan Ahmed ORCID: https://orcid.org/0000-0003-1596-4155, Lian, Yunhe and Sang, Yaxin 2024. Integrated network pharmacology and transcriptomic approach reveal the role of equol in reducing colorectal cancer via regulating multiple cell cycle genes in HCT116 cells. International Journal of Biological Macromolecules 282 (Pt 2) , 136832. 10.1016/j.ijbiomac.2024.136832 Item availability restricted.

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Abstract

Equol is an isoflavone-derived metabolite known to exhibit strong estrogenic and antioxidant activities. The aim of this paper is twofold: first, to confirm the anticancer potential of equol against colorectal cancer, and second, to reveal the underlying mechanisms. After treatment with 40 μg/mL equol, cell proliferation, cell migration, and colony formation of HCT116 colon cancer cells were inhibited. Network pharmacology and transcriptomics analysis revealed the downregulation of genes related to DNA replication (CCND1, E2F1, CDC6, CDC45, MCM4), leading to G1/S cell cycle arrest and the induction of cell apoptosis, which was confirmed by flow cytometry. Genes associated with the G2-to-M transition (CDK1, CCNA2, CCNB1) were also downregulated. In addition, equol downregulated genes (FOXM1 and ASPM) that control cell migration and invasion. Our data indicate that equol can inhibit colorectal cancer by targeting multiple pathways, suggesting its potential as a key component in the adjuvant treatment of colorectal cancer. [Abstract copyright: Copyright © 2024. Published by Elsevier B.V.]

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry
Publisher:	Elsevier
ISSN:	0141-8130
Date of First Compliant Deposit:	12 November 2024
Date of Acceptance:	21 October 2024
Last Modified:	12 Nov 2024 13:15
URI:	https://orca.cardiff.ac.uk/id/eprint/173817

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