T cell receptor recognition of hybrid insulin peptides bound to HLA-DQ8

Tran, Mai T., Faridi, Pouya, Lim, Jia Jia, Ting, Yi Tian, Onwukwe, Goodluck, Bhattacharjee, Pushpak, Jones, Claerwen M., Tresoldi, Eleonora, Cameron, Fergus J., La Gruta, Nicole L., Purcell, Anthony W., Mannering, Stuart I., Rossjohn, Jamie ORCID: https://orcid.org/0000-0002-2020-7522 and Reid, Hugh H. 2021. T cell receptor recognition of hybrid insulin peptides bound to HLA-DQ8. Nature Communications 12 (1) , 5110. 10.1038/s41467-021-25404-x

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Abstract

HLA-DQ8, a genetic risk factor in type I diabetes (T1D), presents hybrid insulin peptides (HIPs) to autoreactive CD4+ T cells. The abundance of spliced peptides binding to HLA-DQ8 and how they are subsequently recognised by the autoreactive T cell repertoire is unknown. Here we report, the HIP (GQVELGGGNAVEVLK), derived from splicing of insulin and islet amyloid polypeptides, generates a preferred peptide-binding motif for HLA-DQ8. HLA-DQ8-HIP tetramer+ T cells from the peripheral blood of a T1D patient are characterised by repeated TRBV5 usage, which matches the TCR bias of CD4+ T cells reactive to the HIP peptide isolated from the pancreatic islets of a patient with T1D. The crystal structure of three TRBV5+ TCR-HLA-DQ8-HIP complexes shows that the TRBV5-encoded TCR β-chain forms a common landing pad on the HLA-DQ8 molecule. The N- and C-termini of the HIP is recognised predominantly by the TCR α-chain and TCR β-chain, respectively, in all three TCR ternary complexes. Accordingly, TRBV5 + TCR recognition of HIP peptides might occur via a ‘polarised’ mechanism, whereby each chain within the αβTCR heterodimer recognises distinct origins of the spliced peptide presented by HLA-DQ8.

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Schools > Medicine
Publisher:	Nature Research
ISSN:	2041-1723
Date of First Compliant Deposit:	7 March 2025
Date of Acceptance:	5 August 2021
Last Modified:	12 Mar 2025 12:45
URI:	https://orca.cardiff.ac.uk/id/eprint/176717

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