Dual-wave hybrid acoustofluidic centrifuge for rapid enrichment of micro- and nanoscale biological particles

Han, Shaoshuai, Wu, Tongfei, Li, Honghao, Li, He, Yang, Xin ORCID: https://orcid.org/0000-0002-7612-614X, Li, Ning, Huang, Tangcheng, Ren, Jun and Wu, Zhenlin 2025. Dual-wave hybrid acoustofluidic centrifuge for rapid enrichment of micro- and nanoscale biological particles. Sensors and Actuators A: Physical 389 , 116495. 10.1016/j.sna.2025.116495 Item availability restricted.

PDF - Accepted Post-Print Version Restricted to Repository staff only until 28 March 2026 due to copyright restrictions. Download (2MB)

Abstract

Rapid enrichment of micro- and nanoscale biological particles in microliter volumes presents substantial potential for applications in biosensing, disease diagnosis, and therapeutic interventions. Herein, a dual-wave hybrid acoustofluidic centrifuge (DHAC) technique is presented, which generates both Rayleigh and Shear Horizontal waves by optimizing the angle between the electrode and the reference flat of the piezoelectric substrate. The dual waves generate a complex acoustic field within the droplet, enabling the effective concentration of nanoparticles and various biological particles. The DHAC efficiently concentrates micro- and nanoscale particles ranging from 50 nm to 1 m within a 150 L sample in 120 s, operating at 34.8 MHz with a chip power of 31 dBm, achieving an enrichment rate approximately 3.6 times higher than that of a single-wave acoustofluidic centrifuge. Furthermore, the cell viability of 293-F cells after 1 h of acoustic excitation in the DHAC device exceeded 90%, further confirming its high biocompatibility. This technology offers significant potential as an advanced tool for precise biochemical analysis and effective disease diagnostics.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Engineering
Publisher:	Elsevier
ISSN:	0924-4247
Date of First Compliant Deposit:	13 May 2025
Date of Acceptance:	18 March 2025
Last Modified:	15 May 2025 11:45
URI:	https://orca.cardiff.ac.uk/id/eprint/178246

Actions (repository staff only)

Edit Item