Analyzing DNA methyltransferase enzymatic activity: Basic methods and applications

Jurkowska, Renata Z. ORCID: https://orcid.org/0000-0002-4507-2222 2025. Analyzing DNA methyltransferase enzymatic activity: Basic methods and applications. Tollefsbol, Trygve O., ed. Epigenetics Methods (Second Edition), Translational Epigenetics, Elsevier, pp. 209-237. (10.1016/B978-0-443-26759-8.00001-1)

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Abstract

DNA methylation plays important biological roles in both prokaryotes and eukaryotes and is often dysregulated in various diseases. DNA methyltransferases (MTases), the enzymes responsible for transferring methyl groups to cytosine or adenine bases in the DNA, have therefore been a focus of extensive studies since their discovery. Hence, there is a continuous effort to develop methods that allow for the investigation of the molecular properties of these fascinating enzymes. Additionally, as DNA MTases are considered attractive therapeutic targets for human diseases, major efforts are made to identify potent and specific compounds that can target these key enzymes. Efficient inhibitor screening requires the development of sensitive, highly accurate, and easily scalable assay formats. This chapter focuses on the most common assays used for the detection of DNA MTase activity in cells and in vitro and for studying their molecular mechanism and specificity. In addition, novel fluorescence- and electrochemical sensing-based assays well-suited for compound screening will be summarized, followed by the description of exciting new biosensors for the real-time tracking of DNA methylation in cells.

Item Type:	Book Section
Date Type:	Published Online
Status:	Published
Schools:	Schools > Biosciences
Publisher:	Elsevier
ISBN:	9780443267598
Last Modified:	15 Oct 2025 09:16
URI:	https://orca.cardiff.ac.uk/id/eprint/181660

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