A mechanism for TCR sharing between T cell subsets and individuals revealed by pyrosequencing

Venturi, Vanessa, Quigley, Maire F., Greenaway, Hui Yee, Ng, Pauline C., Ende, Zachary S., McIntosh, Tina, Asher, Tedi E., Almeida, Jorge R., Levy, Samuel, Price, David ORCID: https://orcid.org/0000-0001-9416-2737, Davenport, Miles P. and Douek, Daniel C. 2011. A mechanism for TCR sharing between T cell subsets and individuals revealed by pyrosequencing. The Journal of Immunology 186 (7) , pp. 4285-4294. 10.4049/jimmunol.1003898

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Abstract

The human naive T cell repertoire is the repository of a vast array of TCRs. However, the factors that shape their hierarchical distribution and relationship with the memory repertoire remain poorly understood. In this study, we used polychromatic flow cytometry to isolate highly pure memory and naive CD8+ T cells, stringently defined with multiple phenotypic markers, and used deep sequencing to characterize corresponding portions of their respective TCR repertoires from four individuals. The extent of interindividual TCR sharing and the overlap between the memory and naive compartments within individuals were determined by TCR clonotype frequencies, such that higher-frequency clonotypes were more commonly shared between compartments and individuals. TCR clonotype frequencies were, in turn, predicted by the efficiency of their production during V(D)J recombination. Thus, convergent recombination shapes the TCR repertoire of the memory and naive T cell pools, as well as their interrelationship within and between individuals.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Systems Immunity Research Institute (SIURI)
Subjects:	Q Science > QR Microbiology > QR180 Immunology
Publisher:	American Association of Immunologists
ISSN:	0022-1767
Last Modified:	20 Oct 2022 08:51
URI:	https://orca.cardiff.ac.uk/id/eprint/29858

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