Extraction and characterization of the rhesus macaque T-cell receptor β-chain genes

Greenaway, Hui Yee, Kurniawan, Monica, Price, David ORCID: https://orcid.org/0000-0001-9416-2737, Douek, Daniel C., Davenport, Miles P. and Venturi, Vanessa 2009. Extraction and characterization of the rhesus macaque T-cell receptor β-chain genes. Immunology and Cell Biology 87 (7) , pp. 546-553. 10.1038/icb.2009.38

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Abstract

Rhesus macaque models have been instrumental in the development and testing of vaccines before human studies and have provided fundamental insights into the determinants of immune efficacy in a variety of infectious diseases. However, the characterization of antigen-specific T-cell receptor (TCR) repertoires during adaptive immune responses in these models has earlier relied on human TCR gene assignments. Here, we extracted and characterized TCR β-chain (TRB) genes from the recently sequenced rhesus macaque genome that are homologous to the human TRB genes. Comparison of the rhesus macaque TRB genes with the human TRB genes showed an average best match similarity of 92.9%. Furthermore, we confirmed the usage of most rhesus macaque TRB genes by expressed TCRβ sequences within epitope-specific TCR repertoires. This primary description of the rhesus macaque TRB genes will provide a standardized nomenclature and enable better characterization of TCR usage in studies that use this species.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Systems Immunity Research Institute (SIURI)
Subjects:	Q Science > QH Natural history > QH426 Genetics Q Science > QR Microbiology > QR180 Immunology
Uncontrolled Keywords:	T-cell, T-cell receptor, T-cell receptor repertoire
Publisher:	Nature Publishing Group
ISSN:	0818-9641
Last Modified:	20 Oct 2022 08:56
URI:	https://orca.cardiff.ac.uk/id/eprint/30085

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