Collagen fibril disorganisation in the corneas of keratocan-deficient mice as revealed by synchrotron X-ray diffraction [Abstract]

Meek, Keith Michael Andrew ORCID: https://orcid.org/0000-0002-9948-7538, Quantock, Andrew James ORCID: https://orcid.org/0000-0002-2484-3120, Akhtar, S., Liu, C. Y. and Kao, W. 2003. Collagen fibril disorganisation in the corneas of keratocan-deficient mice as revealed by synchrotron X-ray diffraction [Abstract]. Investigative Ophthalmology and Visual Science 44 , U245-U245.

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Abstract

Purpose: To examine collagen fibril diameter, spacing and organisation in the corneal stromas of keratocan-null Kera-/- mice. Methods: Immediately after enucleation, the corneas from 4-month-old Kera-/- mice and their age-matched, wild-type littermates were either fixed in 2.5% formalin (n=8) or frozen (n=12) and transported to the synchrotron radiation source at Daresbury, UK. Low-angle synchrotron X-ray patterns were obtained from which the average fibril diameter, interfibrillar spacing and the degree of order in the nearest neighbour fibril packing were assessed. Results:In corneas that were stored frozen, a small but significant increase in the average diameter of the collagen fibrils was found between wild-type and Kera-/- corneas (32.2 nm to 35.3 nm) together with an increase in the average interfibrillar spacing (44.6 nm to 50.7 nm). Kera-/- mice showed a significant decrease in local fibril order. Similar effects were found in the frozen and fixed corneas. Conclusions: Kera-/- corneas showed an increased diameter and interfibrillar spacing suggesting that these corneas were more hydrated. The constituent fibrils were more disordered, an effect also observed in Lum-/- mice (Invest. Ophthalmol. Vis. Sci. 42:1750-1756) although, unlike in Lum-/- mice, it has been previously noted that there is no increase in light scattering in Kera-/- corneas.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Optometry and Vision Sciences
Subjects:	R Medicine > RE Ophthalmology
Uncontrolled Keywords:	cornea: stroma and keratocytes • proteoglycans/glycosaminoglycans • cornea: basic science
Publisher:	Association for Research in Vision and Ophthalmology
ISSN:	0146-0404
Last Modified:	20 Oct 2022 09:53
URI:	https://orca.cardiff.ac.uk/id/eprint/33442

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