White, P. Lewis, Williams, David Wynne  ORCID: https://orcid.org/0000-0002-7351-5131, Kuriyama, Tomoari, Samad, Shamim A., Lewis, Michael Alexander Oxenham ORCID: https://orcid.org/0000-0003-1917-0651 and Barnes, Rosemary Ann
2004.
Detection of Candida in concentrated oral rinse cultures by real-time PCR.
Journal of clinical microbiology
42
(5)
, pp. 2101-2107.
10.1128/JCM.42.5.2101-2107.2004
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Abstract
The incidence of oral candidosis has increased in recent years, largely as a result of the emergence of human immunodeficiency virus infection and the more widespread use of immunosuppressive chemotherapy. This development has been associated with a need for more reliable methods for the detection of Candida. The present study assessed the performance of a real-time PCR and two block-based PCRs for the detection of Candida in 193 concentrated oral rinse culture (CRC) specimens. A total of 102 CRC specimens were positive by culture for Candida; and 96, 90, and 75 of these were also positive by real-time, N18-specific, and internal transcribed spacer (ITS)-specific PCRs, respectively. The five false-negative results by the real-time PCR were all non-Candida albicans positive by culture. Of the 91 culture-negative CRC specimens, 20, 41, and 44 were positive by the real-time PCR and the N18- and ITS-specific PCRs, respectively. All three PCRs detected fungal DNA in 8 culture-negative CRC specimens, with a further 30 being positive by two of the three PCRs. A total of 32 CRC specimens were Candida free by all methods. In summary, a real-time PCR that provides a sensitive, specific, and rapid alternative technique for detection of Candida in the mouth is described.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Medicine Schools > Dentistry |
| Additional Information: | Pdf uploaded in accordance with publisher's policy at http://www.sherpa.ac.uk/romeo/issn/0095-1137/ (accessed 25/02/2014) |
| Publisher: | American Society for Microbiology |
| ISSN: | 0095-1137 |
| Date of First Compliant Deposit: | 30 March 2016 |
| Last Modified: | 01 Nov 2023 07:40 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/335 |
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