Detection of Candida in concentrated oral rinse cultures by real-time PCR

White, P. Lewis, Williams, David Wynne ORCID: https://orcid.org/0000-0002-7351-5131, Kuriyama, Tomoari, Samad, Shamim A., Lewis, Michael Alexander Oxenham ORCID: https://orcid.org/0000-0003-1917-0651 and Barnes, Rosemary Ann 2004. Detection of Candida in concentrated oral rinse cultures by real-time PCR. Journal of clinical microbiology 42 (5) , pp. 2101-2107. 10.1128/JCM.42.5.2101-2107.2004

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Abstract

The incidence of oral candidosis has increased in recent years, largely as a result of the emergence of human immunodeficiency virus infection and the more widespread use of immunosuppressive chemotherapy. This development has been associated with a need for more reliable methods for the detection of Candida. The present study assessed the performance of a real-time PCR and two block-based PCRs for the detection of Candida in 193 concentrated oral rinse culture (CRC) specimens. A total of 102 CRC specimens were positive by culture for Candida; and 96, 90, and 75 of these were also positive by real-time, N18-specific, and internal transcribed spacer (ITS)-specific PCRs, respectively. The five false-negative results by the real-time PCR were all non-Candida albicans positive by culture. Of the 91 culture-negative CRC specimens, 20, 41, and 44 were positive by the real-time PCR and the N18- and ITS-specific PCRs, respectively. All three PCRs detected fungal DNA in 8 culture-negative CRC specimens, with a further 30 being positive by two of the three PCRs. A total of 32 CRC specimens were Candida free by all methods. In summary, a real-time PCR that provides a sensitive, specific, and rapid alternative technique for detection of Candida in the mouth is described.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Dentistry
Additional Information:	Pdf uploaded in accordance with publisher's policy at http://www.sherpa.ac.uk/romeo/issn/0095-1137/ (accessed 25/02/2014)
Publisher:	American Society for Microbiology
ISSN:	0095-1137
Date of First Compliant Deposit:	30 March 2016
Last Modified:	01 Nov 2023 07:40
URI:	https://orca.cardiff.ac.uk/id/eprint/335

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