Brilliant violet fluorophores: A new class of ultrabright fluorescent compounds for immunofluorescence experiments

Chattopadhyay, Pratip K., Gaylord, Brent, Palmer, Adrian, Jiang, Nan, Raven, Mary A., Lewis, Geoff, Reuter, Morgan A., Nur-ur Rahman, A. K. M., Price, David ORCID: https://orcid.org/0000-0001-9416-2737, Betts, Michael R. and Roederer, Mario 2012. Brilliant violet fluorophores: A new class of ultrabright fluorescent compounds for immunofluorescence experiments. Cytometry Part A 81A (6) , pp. 456-466. 10.1002/cyto.a.22043

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Abstract

The Nobel Prize in Chemistry was awarded in 2000 for the discovery of conductive organic polymers, which have subsequently been adapted for applications in ultrasensitive biological detection. Here, we report the first use of this new class of fluorescent probes in a diverse range of cytometric and imaging applications. We demonstrate that these “Brilliant Violet” reporters are dramatically brighter than other UV-violet excitable dyes, and are of similar utility to phycoerythrin (PE) and allophycocyanin (APC). They are thus ideally suited for cytometric assays requiring high sensitivity, such as MHC-multimer staining or detection of intracellular antigens. Furthermore, these reporters are sensitive and spectrally distinct options for fluorescence imaging, two-photon microscopy and imaging cytometry. These ultra-bright materials provide the first new high-sensitivity fluorescence probes in over 25 years and will have a dramatic impact on the design and implementation of multicolor panels for high-sensitivity immunofluorescence assays.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Systems Immunity Research Institute (SIURI)
Subjects:	Q Science > QD Chemistry R Medicine > R Medicine (General)
Uncontrolled Keywords:	brilliant violet; fluorochromes; dyes; immunofluorescence; violet laser; conductive polymers
Publisher:	Wiley-Blackwell
ISSN:	1552-4922
Last Modified:	21 Oct 2022 10:49
URI:	https://orca.cardiff.ac.uk/id/eprint/41449

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