Duffy, Margaret R., Bradshaw, Angela C., Parker, Alan L.  ORCID: https://orcid.org/0000-0002-9302-1761, McVey, John H. and Baker, Andrew H.
2011.
A Cluster of Basic Amino Acids in the Factor X Serine Protease Mediates Surface Attachment of Adenovirus/FX Complexes.
Journal of Virology
85
(20)
, pp. 10914-10919.
10.1128/JVI.05382-11
|
Preview |
PDF
- Published Version
Download (1MB) | Preview |
Abstract
Hepatocyte transduction following intravenous administration of adenovirus 5 (Ad5) is mediated by interaction between coagulation factor X (FX) and the hexon. The FX serine protease (SP) domain tethers the Ad5/FX complex to hepatocytes through binding heparan sulfate proteoglycans (HSPGs). Here, we identify the critical HSPG-interacting residues of FX. We generated an FX mutant by modifying seven residues in the SP domain. Surface plasmon resonance demonstrated that mutations did not affect binding to Ad5. FX-mediated, HSPG-associated cell binding and transduction were abolished. A cluster of basic amino acids in the SP domain therefore mediates surface interaction of the Ad/FX complex.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Medicine |
| Subjects: | Q Science > QR Microbiology > QR355 Virology R Medicine > RM Therapeutics. Pharmacology |
| Additional Information: | Pdf uploaded in accordance with publisher's policy at http://www.sherpa.ac.uk/romeo/issn/0022-538X/ (accessed 25/02/2014) |
| Publisher: | American Society for Microbiology |
| ISSN: | 0022-538X |
| Date of First Compliant Deposit: | 30 March 2016 |
| Last Modified: | 11 May 2023 07:21 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/43280 |
Citation Data
Cited 31 times in Scopus. View in Scopus. Powered By Scopus® Data
Actions (repository staff only)
 |
Edit Item |
Altmetric
Altmetric