Considerations for accurate gene expression measurement by reverse transcription quantitative PCR when analysing clinical samples

Sanders, Rebecca, Mason, Deborah Jane ORCID: https://orcid.org/0000-0002-8666-6094, Foy, Carole A. and Huggett, Jim F. 2014. Considerations for accurate gene expression measurement by reverse transcription quantitative PCR when analysing clinical samples. Analytical and Bioanalytical Chemistry 406 (26) , pp. 6471-6483. 10.1007/s00216-014-7857-x

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Abstract

Reverse transcription quantitative PCR is an established, simple and effective method for RNA measurement. However, technical standardisation challenges combined with frequent insufficient experimental detail render replication of many published findings challenging. Consequently, without adequate consideration of experimental standardisation, such findings may be sufficient for a given publication but cannot be translated to wider clinical application. This article builds on earlier standardisation work and the MIQE guidelines, discussing processes that need consideration for accurate, reproducible analysis when dealing with patient samples. By applying considerations common to the science of measurement (metrology), one can maximise the impact of gene expression studies, increasing the likelihood of their translation to clinical tools.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Subjects:	Q Science > Q Science (General) R Medicine > R Medicine (General)
Uncontrolled Keywords:	RNA; uncertainty; normalisation; reverse transcription quantitative PCR. Standardisation.
Publisher:	Springer Verlag
ISSN:	1618-2642
Date of First Compliant Deposit:	18 February 2019
Last Modified:	23 May 2023 17:33
URI:	https://orca.cardiff.ac.uk/id/eprint/61331

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