A reproducible method for the expansion of mouse CD8+ T lymphocytes

Lewis, Mark David, de Leenheer, Evy, Fishman, Sigal, Siew, Lai Khai, Gross, Gideon and Wong, Florence Susan ORCID: https://orcid.org/0000-0002-2812-8845 2015. A reproducible method for the expansion of mouse CD8+ T lymphocytes. Journal of Immunological Methods 417 , pp. 134-138. 10.1016/j.jim.2015.01.004

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Abstract

Murine adoptive CD8 + T-cell immunotherapy studies require the generation of large numbers of high viability CD8 + cells. Here we report a tissue culture protocol for the reliable expansion of CD8 + T-cells derived from murine spleen to give a 20-fold expansion after 4 days in culture. The cells were transfected with an mRNA GFP construct and transferred into NOD mice. GFP positive cells could be detected 7 days after transfer thus confirming that the cells survive and are functional for up to 1 week.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Systems Immunity Research Institute (SIURI)
Subjects:	R Medicine > R Medicine (General)
Publisher:	Elsevier
ISSN:	0022-1759
Funders:	MRC
Date of First Compliant Deposit:	30 March 2016
Date of Acceptance:	13 January 2015
Last Modified:	16 May 2023 21:32
URI:	https://orca.cardiff.ac.uk/id/eprint/72759

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