Jiang, Wen Guo  ORCID: https://orcid.org/0000-0002-3283-1111, Puntis, M.C.A. and Hallett, Maurice Bartlett ORCID: https://orcid.org/0000-0001-8197-834X
1992.
U937 cells stimulated with opsonised zymozan particles provide a convenient laboratory source of tumour necrosis factor α.
Journal of Immunological Methods
152
(2)
, pp. 201-207.
10.1016/0022-1759(92)90141-F
|
Abstract
The U937 cell line has been shown to generate tumour fibrosis factor α (TNF-α) in response to soluble stimuli such as PMA and LPS, but only after treatment with GM-CSF. We report here the generation of TNF-α from U937 cells following phagocytosis of opsonised zymozan particles without the need for pre-treatment with GM CSF. The release of TNF-α from U937 cells was demonstrated by a specific radioimmunoassay, L929 cell killing and neutrophil ‘priming’. The biological activities in the cell supernatant were inhibited by TNF-α affarserum. Phagocytosis was required for TNF-α production. Non-opsonised zymozan or latex particles which were not phagocytosed or pretreatment with cytochalasin B, which inhibited phagocytosis of opsonised zymozan particles, all failed to trigger TNF-α production. Phagocytosis failed to trigger detectable IL-1 generation, and production of IL-6 was insufficient to produce biological effects on neutrophils. The U937 supernatant thus provides a source of human TNF-α which can be generated conveniently and cheaply for experimental investigations.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Medicine |
| Subjects: | R Medicine > R Medicine (General) |
| Publisher: | Elsevier |
| ISSN: | 0022-1759 |
| Last Modified: | 28 Oct 2022 09:01 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/72999 |
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