U937 cells stimulated with opsonised zymozan particles provide a convenient laboratory source of tumour necrosis factor α

Jiang, Wen Guo ORCID: https://orcid.org/0000-0002-3283-1111, Puntis, M.C.A. and Hallett, Maurice Bartlett ORCID: https://orcid.org/0000-0001-8197-834X 1992. U937 cells stimulated with opsonised zymozan particles provide a convenient laboratory source of tumour necrosis factor α. Journal of Immunological Methods 152 (2) , pp. 201-207. 10.1016/0022-1759(92)90141-F

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Abstract

The U937 cell line has been shown to generate tumour fibrosis factor α (TNF-α) in response to soluble stimuli such as PMA and LPS, but only after treatment with GM-CSF. We report here the generation of TNF-α from U937 cells following phagocytosis of opsonised zymozan particles without the need for pre-treatment with GM CSF. The release of TNF-α from U937 cells was demonstrated by a specific radioimmunoassay, L929 cell killing and neutrophil ‘priming’. The biological activities in the cell supernatant were inhibited by TNF-α affarserum. Phagocytosis was required for TNF-α production. Non-opsonised zymozan or latex particles which were not phagocytosed or pretreatment with cytochalasin B, which inhibited phagocytosis of opsonised zymozan particles, all failed to trigger TNF-α production. Phagocytosis failed to trigger detectable IL-1 generation, and production of IL-6 was insufficient to produce biological effects on neutrophils. The U937 supernatant thus provides a source of human TNF-α which can be generated conveniently and cheaply for experimental investigations.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Publisher:	Elsevier
ISSN:	0022-1759
Last Modified:	28 Oct 2022 09:01
URI:	https://orca.cardiff.ac.uk/id/eprint/72999

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