Analysis of DGGE profiles to explore the relationship between prokaryotic community composition and biogeochemical processes in deep subseafloor sediments from the Peru Margin

Fry, John Christopher, Webster, Gordon ORCID: https://orcid.org/0000-0002-9530-7835, Cragg, Barry Andrew, Weightman, Andrew John ORCID: https://orcid.org/0000-0002-6671-2209 and Parkes, Ronald John 2006. Analysis of DGGE profiles to explore the relationship between prokaryotic community composition and biogeochemical processes in deep subseafloor sediments from the Peru Margin. FEMS Microbiology Ecology 58 (1) , pp. 86-98. 10.1111/j.1574-6941.2006.00144.x

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Abstract

The aim of this work was to relate depth profiles of prokaryotic community composition with geochemical processes in the deep subseafloor biosphere at two shallow-water sites on the Peru Margin in the Pacific Ocean (ODP Leg 201, sites 1228 and 1229). Principal component analysis of denaturing gradient gel electrophoresis banding patterns of deep-sediment Bacteria, Archaea, Euryarchaeota and the novel candidate division JS1, followed by multiple regression, showed strong relationships with prokaryotic activity and geochemistry (R2=55–100%). Further correlation analysis, at one site, between the principal components from the community composition profiles for Bacteria and 12 other variables quantitatively confirmed their relationship with activity and geochemistry, which had previously only been implied. Comparison with previously published cell counts enumerated by fluorescent in situ hybridization with rRNA-targeted probes confirmed that these denaturing gradient gel electrophoresis profiles described an active prokaryotic community

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Earth and Environmental Sciences
Uncontrolled Keywords:	Prokaryotic biodiversity ; Community composition ; Geochemistry ; Deep biosphere ; Denaturing gradient gel electrophoresis profiles ; Statistical analysis
Publisher:	Wiley-Blackwell
ISSN:	0168-6496
Last Modified:	15 Nov 2024 22:31
URI:	https://orca.cardiff.ac.uk/id/eprint/7551

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