Modified linker-PCR primers facilitate complete sequencing of DGGE DNA fragments

O'Sullivan, Louise Anne, Webster, Gordon ORCID: https://orcid.org/0000-0002-9530-7835, Fry, John Christopher, Parkes, Ronald John and Weightman, Andrew ORCID: https://orcid.org/0000-0002-6671-2209 2008. Modified linker-PCR primers facilitate complete sequencing of DGGE DNA fragments. Journal of Microbiological Methods 75 (3) , pp. 579-581. 10.1016/j.mimet.2008.08.006

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Abstract

Modified linker-PCR primers were developed to enable complete sequencing of a DGGE band in one reaction. Commonly used bacterial and archaeal 16S rRNA gene PCR-DGGE primers were modified to contain linkers and sequencing primers. This protocol does not involve additional stages, and improves retrieval of sequence from DGGE bands by ≈23%.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Schools > Biosciences Schools > Earth and Environmental Sciences
Subjects:	Q Science > QR Microbiology
Uncontrolled Keywords:	PCR-DGGE; 16S rRNA genes; Prokaryotic diversity; Bacteria Archaea
Publisher:	Elsevier
ISSN:	0167-7012
Last Modified:	15 Nov 2024 22:33
URI:	https://orca.cardiff.ac.uk/id/eprint/8642

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