Ashelford, Kevin E. ![]() |
Official URL: http://dx.doi.org/10.1186/gb-2011-12-3-r28
Abstract
Map based cloning in Arabidopsis thaliana can be a difficult and time-consuming process, specifically if the phenotype is subtle and scoring labour intensive. Here, we have re-sequenced the 120-Mb genome of a novel Arabidopsis clock mutant early bird (ebi-1) in Wassilewskija (Ws-2). We demonstrate the utility of sequencing a backcrossed line in limiting the number of SNPs considered. We identify a SNP in the gene AtNFXL-2 as the likely cause of the ebi-1 phenotype.
Item Type: | Article |
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Date Type: | Publication |
Status: | Published |
Schools: | Medicine |
Subjects: | Q Science > QH Natural history > QH426 Genetics Q Science > QK Botany |
Publisher: | Genome Biology |
ISSN: | 1465-6906 |
Funders: | European Commision: Marie Skłodowska-Curie Actions, Swedish Research Council, Swedish Foundation for Strategic Research, Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning, Swedish Governmental Agency for Innovation Systems (VINNOVA), University of Liverpool, Biotechnology and Biological Sciences Research Council, Engineering and Physical Sciences Research Council, Royal Society |
Date of Acceptance: | 23 March 2011 |
Last Modified: | 31 Oct 2022 10:55 |
URI: | https://orca.cardiff.ac.uk/id/eprint/86711 |
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