Kille, Peter  ORCID: https://orcid.org/0000-0001-6023-5221, Morgan, Andrew, Kate, Powell, Frederick, Mosselmans, Daniel, Hart, Paul, Gunning, Hayes, Anthony Joseph, Scarborough, Derek, McDonald, Iain ORCID: https://orcid.org/0000-0001-9066-7244 and John, Charnock
2016.
Venus trapped, Mars transits: Cu and Fe redox chemistry, cellular topography, and in situ ligand binding in terrestrial isopod hepatopancreas.
Open Biology Journal
6
(3)
, -.
10.1098/rsob.150270
|
Preview |
PDF
- Published Version
Available under License Creative Commons Attribution. Download (1MB) | Preview |
Abstract
Woodlice efficiently sequester copper (Cu) in ‘cuprosomes' within hepatopancreatic ‘S' cells. Binuclear ‘B’ cells in the hepatopancreas form iron (Fe) deposits; these cells apparently undergo an apocrine secretory diurnal cycle linked to nocturnal feeding. Synchrotron-based µ-focus X-ray spectroscopy undertaken on thin sections was used to characterize the ligands binding Cu and Fe in S and B cells of Oniscus asellus (Isopoda). Main findings were: (i) morphometry confirmed a diurnal B-cell apocrine cycle; (ii) X-ray fluorescence (XRF) mapping indicated that Cu was co-distributed with sulfur (mainly in S cells), and Fe was co-distributed with phosphate (mainly in B cells); (iii) XRF mapping revealed an intimate morphological relationship between the basal regions of adjacent S and B cells; (iv) molecular modelling and Fourier transform analyses indicated that Cu in the reduced Cu+ state is mainly coordinated to thiol-rich ligands (Cu–S bond length 2.3 Å) in both cell types, while Fe in the oxidized Fe3+ state is predominantly oxygen coordinated (estimated Fe–O bond length of approx. 2 Å), with an outer shell of Fe scatterers at approximately 3.05 Å; and (v) no significant differences occur in Cu or Fe speciation at key nodes in the apocrine cycle. Findings imply that S and B cells form integrated unit-pairs; a functional role for secretions from these cellular units in the digestion of recalcitrant dietary components is hypothesized.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Earth and Environmental Sciences Schools > Biosciences |
| Publisher: | Bentham Open / Bentham open |
| ISSN: | 1874-1967 |
| Funders: | STFC |
| Date of First Compliant Deposit: | 30 March 2016 |
| Date of Acceptance: | 9 February 2016 |
| Last Modified: | 20 May 2023 07:30 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/86957 |
Citation Data
Cited 2 times in Scopus. View in Scopus. Powered By Scopus® Data
Actions (repository staff only)
 |
Edit Item |
Dimensions
Dimensions