Innate recognition of bacteria in human milk is mediated by a milk-derived highly expressed pattern recognition receptor, soluble CD14

Labeta, Mario ORCID: https://orcid.org/0000-0001-5750-6983, Vidal, K., Rey Nores, J. E., Arias, M., Vita, N., Morgan, Bryan Paul ORCID: https://orcid.org/0000-0003-4075-7676, Guillemot, J.C., Loyaux, D., Ferrara, P., Schmid, D., Affolter, M., Borysiewicz, L. K., Donnet-Hughes, A. and Schiffrin, E. J. 2000. Innate recognition of bacteria in human milk is mediated by a milk-derived highly expressed pattern recognition receptor, soluble CD14. Journal of Experimental Medicine 191 , pp. 1807-1812. 10.1084/jem.191.10.1807

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Abstract

Little is known about innate immunity to bacteria after birth in the hitherto sterile fetal intestine. Breast-feeding has long been associated with a lower incidence of gastrointestinal infections and inflammatory and allergic diseases. We found in human breast milk a 48-kD polypeptide, which we confirmed by mass spectrometry and sequencing to be a soluble form of the bacterial pattern recognition receptor CD14 (sCD14). Milk sCD14 (m-sCD14) concentrations were up to 20-fold higher than serum sCD14 from nonpregnant, pregnant, or lactating women. In contrast, lipopolysaccharide (LPS)-binding protein was at very low levels. Mammary epithelial cells produced 48-kD sCD14. m-sCD14 mediated activation by LPS and whole bacteria of CD14 negative cells, including intestinal epithelial cells, resulting in release of innate immune response molecules. m-sCD14 was undetectable in the infant formulas and commercial (cows') milk tested, although it was present in bovine colostrum. These findings indicate a sentinel role for sCD14 in human milk during bacterial colonization of the gut, and suggest that m-sCD14 may be involved in modulating local innate and adaptive immune responses, thus controlling homeostasis in the neonatal intestine.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Publisher:	Rockefeller University Press
Date of First Compliant Deposit:	1 July 2016
Date of Acceptance:	3 March 2000
Last Modified:	03 May 2023 18:50
URI:	https://orca.cardiff.ac.uk/id/eprint/92254

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