Use of the Dictyostelium stalk cell assay to monitor GSK-3 regulation

Harwood, Adrian John ORCID: https://orcid.org/0000-0003-3124-5169 2008. Use of the Dictyostelium stalk cell assay to monitor GSK-3 regulation. Vincan, Elizabeth, ed. Wnt Signaling, Vol. 469. Methods in Molecular Biology, Springer, pp. 39-43. (10.1007/978-1-60327-469-2_4)

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Abstract

GSK-3 activity mediates cAMP repression of stalk induction of cells in low-density monolayer culture. The lower the GSK-3 activity the greater the percentage of stalk cells formed. This protocol describes a robust and quantitative method utilizing an adapted stalk cell monolayer assay to measure GSK-3 activation.

Item Type:	Book Section
Date Type:	Publication
Status:	Published
Schools:	Biosciences Neuroscience and Mental Health Research Institute (NMHRI)
Subjects:	Q Science > QR Microbiology
Publisher:	Springer
ISBN:	9781603274685
ISSN:	1064-3745
Last Modified:	18 Oct 2022 12:17
URI:	https://orca.cardiff.ac.uk/id/eprint/9229

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