Yang, Yang, Wang, Hexiao, Zhang, Xinrui, Huo, Wei, Qi, Ruiqun, Gao, Yali, Zhang, Gaofeng, Song, Bing  ORCID: https://orcid.org/0000-0001-9356-2333, Chen, Hongduo and Gao, Xinghua
2017.
Heat increases the editing efficiency of human papillomavirus E2 gene by inducing upregulation of APOBEC3A and 3G.
Journal of Investigative Dermatology
137
(4)
, pp. 810-818.
10.1016/j.jid.2016.06.635
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Abstract
Apolipoprotein B mRNA-editing catalytic polypeptide (APOBEC) 3 proteins have been identified as potent viral DNA mutators and have broad antiviral activity. In this study, we demonstrated that apolipoprotein B mRNA-editing catalytic polypeptide 3A (A3A) and A3G expression levels were significantly upregulated in human papillomavirus (HPV)-infected cell lines and tissues. Heat treatment resulted in elevated expression of A3A and A3G in a temperature-dependent manner in HPV-infected cells. Correspondingly, HPV-infected cells heat-treated at 44 °C showed accumulated G-to-A or C-to-T mutation in HPV E2 gene. Knockdown of A3A or A3G could promote cell viability, along with the lower frequency of A/T in HPV E2 gene. In addition, regressing genital viral warts also harbored high G-to-A or C-to-T mutation in HPV E2 gene. Taken together, we demonstrate that apolipoprotein B mRNA-editing catalytic polypeptide 3 expression and editing function was heat sensitive to a certain degree, partly explaining the mechanism of action of local hyperthermia to treat viral warts.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Dentistry |
| Subjects: | R Medicine > RK Dentistry |
| Publisher: | Elsevier |
| ISSN: | 0022-202X |
| Date of First Compliant Deposit: | 7 July 2016 |
| Date of Acceptance: | 30 June 2016 |
| Last Modified: | 06 Nov 2024 21:00 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/92404 |
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