Heat increases the editing efficiency of human papillomavirus E2 gene by inducing upregulation of APOBEC3A and 3G

Yang, Yang, Wang, Hexiao, Zhang, Xinrui, Huo, Wei, Qi, Ruiqun, Gao, Yali, Zhang, Gaofeng, Song, Bing ORCID: https://orcid.org/0000-0001-9356-2333, Chen, Hongduo and Gao, Xinghua 2017. Heat increases the editing efficiency of human papillomavirus E2 gene by inducing upregulation of APOBEC3A and 3G. Journal of Investigative Dermatology 137 (4) , pp. 810-818. 10.1016/j.jid.2016.06.635

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Abstract

Apolipoprotein B mRNA-editing catalytic polypeptide (APOBEC) 3 proteins have been identified as potent viral DNA mutators and have broad antiviral activity. In this study, we demonstrated that apolipoprotein B mRNA-editing catalytic polypeptide 3A (A3A) and A3G expression levels were significantly upregulated in human papillomavirus (HPV)-infected cell lines and tissues. Heat treatment resulted in elevated expression of A3A and A3G in a temperature-dependent manner in HPV-infected cells. Correspondingly, HPV-infected cells heat-treated at 44 °C showed accumulated G-to-A or C-to-T mutation in HPV E2 gene. Knockdown of A3A or A3G could promote cell viability, along with the lower frequency of A/T in HPV E2 gene. In addition, regressing genital viral warts also harbored high G-to-A or C-to-T mutation in HPV E2 gene. Taken together, we demonstrate that apolipoprotein B mRNA-editing catalytic polypeptide 3 expression and editing function was heat sensitive to a certain degree, partly explaining the mechanism of action of local hyperthermia to treat viral warts.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry
Subjects:	R Medicine > RK Dentistry
Publisher:	Elsevier
ISSN:	0022-202X
Date of First Compliant Deposit:	7 July 2016
Date of Acceptance:	30 June 2016
Last Modified:	06 Nov 2024 21:00
URI:	https://orca.cardiff.ac.uk/id/eprint/92404

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