Three- and four-dimensional visualization of cell migration using optical coherence tomography

Rey, Sara Maria, Považay, Boris, Hofer, Bernd, Unterhuber, A., Hermann, Boris ORCID: https://orcid.org/0000-0001-8658-485X, Harwood, Adrian John ORCID: https://orcid.org/0000-0003-3124-5169 and Drexler, Wolfgang 2009. Three- and four-dimensional visualization of cell migration using optical coherence tomography. Journal of Biophotonics 2 (6-7) , pp. 370-379. 10.1002/jbio.200910027

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Abstract

Conventionally, cell chemotaxis is studied on two-dimensional (2D) transparent surfaces, due to limitations in optical and image data-collection techniques. However, surfaces that more closely mimic the natural environment of cells are often opaque. Optical coherence tomography (OCT) is a noninvasive label-free imaging technique, which offers the potential to visualize moving cells on opaque surfaces and in three dimensions (3D). Here, we demonstrate that OCT is an effective means of time-lapse videomicroscopy of Dictyostelium cells undergoing 3D (2D+time) cell migration on nitrocellulose substrates and 4D (3D+time) chemotaxis within low-density agarose gels. The generated image sequences are compatible with current computer-based image-analysis software for quantification of cell motility. This demonstrates the utility of OCT for cell tracking and analysis of cell chemotaxis in complex environments. (© 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Optometry and Vision Sciences Biosciences Neuroscience and Mental Health Research Institute (NMHRI)
Subjects:	Q Science > QC Physics Q Science > QH Natural history > QH301 Biology Q Science > QR Microbiology
Uncontrolled Keywords:	Optical coherence tomography; noninvasive imaging; cell migration; chemotaxis
Publisher:	Wiley-Blackwell
ISSN:	1864-063X
Last Modified:	18 Oct 2022 12:17
URI:	https://orca.cardiff.ac.uk/id/eprint/9260

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