Antimicrobial activity of Manuka honey against antibiotic resistant strains of the cell wall free bacteria ureaplasma parvum and Ureaplasma urealyticum

Hillitt, K. L., Jenkins, R. E., Spiller, Owen Bradley ORCID: https://orcid.org/0000-0002-9117-6911 and Beeton, M. L. 2017. Antimicrobial activity of Manuka honey against antibiotic resistant strains of the cell wall free bacteria ureaplasma parvum and Ureaplasma urealyticum. Letters In Applied Microbiology 64 (3) , pp. 198-202. 10.1111/lam.12707

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Abstract

The susceptibility of the cell-wall free bacterial pathogens Ureaplasma spp. to Manuka honey was examined. The minimum inhibitory concentration (MIC) of Manuka honey for four Ureaplasma urealyticum and four Ureaplasma parvum isolates was determined. Sensitivity to honey was also compared to clinical isolates with resistance to tetracycline, macrolide and fluoroquinolone antibiotics. Finally step-wise resistance training was utilised in an attempt to induce increased tolerance to honey. The MIC was dependent on the initial bacterial load with 7.5 % and 18.0 % w/v honey required to inhibit U. urealyticum at 1 and 10 6 colour changing units (CCU), respectively, and 4.8 % and 15.3 % w/v required to inhibit U. parvum at 1 and 10 6 CCU, respectively. MIC values were consistently lower for U. parvum compared with U. urealyticum. Antimicrobial activity was seen against tetracycline resistant, erythromycin resistant and ciprofloxacin resistant isolates at 10 5 CCU. No resistance to honey was observed with fifty consecutive challenges at increasing concentrations of honey. This is the first report of the antimicrobial activity of Manuka honey against a cell-wall free bacterial pathogen. The antimicrobial activity was retained against antibiotic resistant strains and it was not possible to generate resistant mutants.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Uncontrolled Keywords:	Antimicrobials, Microbial structure, Infection, Microbial physiology, Resistance
Publisher:	Blackwell Publishing
ISSN:	0266-8254
Date of First Compliant Deposit:	5 January 2017
Date of Acceptance:	13 December 2016
Last Modified:	07 Nov 2023 04:03
URI:	https://orca.cardiff.ac.uk/id/eprint/97184

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