Intranasal administration of RSV antigen-expressing MCMV elicits robust tissue-resident effector and effector memory CD8+ T cells in the lung

Morabito, K M, Ruckwardt, T R, Redwood, A J, Moin, S M, Price, David ORCID: https://orcid.org/0000-0001-9416-2737 and Graham, B S 2017. Intranasal administration of RSV antigen-expressing MCMV elicits robust tissue-resident effector and effector memory CD8+ T cells in the lung. Mucosal Immunology 10 (2) , pp. 545-554. 10.1038/mi.2016.48

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Abstract

Cytomegalovirus vectors are promising delivery vehicles for vaccine strategies that aim to elicit effector CD8+ T cells. To determine how the route of immunization affects CD8+ T cell responses in the lungs of mice vaccinated with a murine cytomegalovirus vector expressing the respiratory syncytial virus matrix (M) protein, we infected CB6F1 mice via the intranasal or intraperitoneal route and evaluated the M-specific CD8+ T cell response at early and late time points. We found that intranasal vaccination generated robust and durable tissue-resident effector and effector memory CD8+ T cell populations that were undetectable after intraperitoneal vaccination. The generation of these antigen-experienced cells by intranasal vaccination resulted in earlier T cell responses, interferon gamma secretion, and viral clearance after respiratory syncytial virus challenge. Collectively, these findings validate a novel approach to vaccination that emphasizes the route of delivery as a key determinant of immune priming at the site of vulnerability.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Additional Information:	This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License
Publisher:	Nature Publishing Group
ISSN:	1933-0219
Date of First Compliant Deposit:	31 January 2017
Date of Acceptance:	14 April 2016
Last Modified:	05 May 2023 09:50
URI:	https://orca.cardiff.ac.uk/id/eprint/97896

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