Morabito, K M, Ruckwardt, T R, Redwood, A J, Moin, S M, Price, David  ORCID: https://orcid.org/0000-0001-9416-2737 and Graham, B S
2017.
Intranasal administration of RSV antigen-expressing MCMV elicits robust tissue-resident effector and effector memory CD8+ T cells in the lung.
Mucosal Immunology
10
(2)
, pp. 545-554.
10.1038/mi.2016.48
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Abstract
Cytomegalovirus vectors are promising delivery vehicles for vaccine strategies that aim to elicit effector CD8+ T cells. To determine how the route of immunization affects CD8+ T cell responses in the lungs of mice vaccinated with a murine cytomegalovirus vector expressing the respiratory syncytial virus matrix (M) protein, we infected CB6F1 mice via the intranasal or intraperitoneal route and evaluated the M-specific CD8+ T cell response at early and late time points. We found that intranasal vaccination generated robust and durable tissue-resident effector and effector memory CD8+ T cell populations that were undetectable after intraperitoneal vaccination. The generation of these antigen-experienced cells by intranasal vaccination resulted in earlier T cell responses, interferon gamma secretion, and viral clearance after respiratory syncytial virus challenge. Collectively, these findings validate a novel approach to vaccination that emphasizes the route of delivery as a key determinant of immune priming at the site of vulnerability.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Medicine |
| Subjects: | R Medicine > R Medicine (General) |
| Additional Information: | This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License |
| Publisher: | Nature Publishing Group |
| ISSN: | 1933-0219 |
| Date of First Compliant Deposit: | 31 January 2017 |
| Date of Acceptance: | 14 April 2016 |
| Last Modified: | 05 May 2023 09:50 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/97896 |
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