FeS-induced radical formation and its effect on plasmid DNA

Rickard, David Terence ORCID: https://orcid.org/0000-0002-4632-5711, Hatton, Bryan, Murphy, Damien Martin ORCID: https://orcid.org/0000-0002-5941-4879, Butler, Ian, Oldroyd, Anthony and Hann, Anthony 2011. FeS-induced radical formation and its effect on plasmid DNA. Aquatic Geochemistry 17 (4-5) , pp. 545-566. 10.1007/s10498-010-9116-x

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Abstract

Plasmid DNA was incubated at 25°C with aqueous solutions of dissolved Fe(II), S(-II), and nanoparticulate FeS with a mackinawite structure, FeSm. At ≥0.1 mM total dissolved Fe(II) and S(-II), an increase in the proportion of the relaxed plasmid DNA occurs, through scission of the DNA backbone. In solutions where FeSm was precipitated, nanoparticulate FeSm binds to the DNA molecules. In solutions with concentrations below the FeSm solubility product, nicking of supercoiled pDNA occurs. Plasmid DNA appears to be a sensitive proxy for radical reactions. The reactant is proposed to be a sulfur-based radical produced from the iron-catalyzed decomposition of bisulfide, in a manner analogous to the Fenton reaction. This is further supported by experiments that suggest that sulfide free radicals are produced during the photolysis of aqueous solutions of polysulfides. Supercoiling of DNA affects nearly all DNA–protein transactions so the observation of relaxation of supercoiled forms through reaction with FeS solutions has direct implications to biochemistry. The results of this experimentation suggest that genotoxicity in FeS-rich systems is a further contributory factor to the limited survival of organisms in sulfidic environments. Mutations resulting from the interactions of organisms and mobile elements, such as plasmids, in sediments will also be affected in sulfide-rich environments.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences Chemistry Earth and Environmental Sciences
Subjects:	Q Science > QD Chemistry Q Science > QE Geology
Uncontrolled Keywords:	Iron sulfide – DNA – Sulfide – Free radicals
Publisher:	Springer
ISSN:	1380-6165
Last Modified:	18 Oct 2022 12:30
URI:	https://orca.cardiff.ac.uk/id/eprint/10067

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