Horizontal transfer of the blaNDM-1 gene to Pseudomonas aeruginosa and Acinetobacter baumannii in biofilms

Tanner, Windy D, Atkinson, Robyn M, Goel, Ramesh K., Toleman, Mark A. ORCID: https://orcid.org/0000-0002-9497-0512, Benson, Lowell Scott, Porucznik, Christina A. and VanDerslice, James A. 2017. Horizontal transfer of the blaNDM-1 gene to Pseudomonas aeruginosa and Acinetobacter baumannii in biofilms. FEMS Microbiology Letters 364 (8) , pp. 102-120. 10.1093/femsle/fnx048

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Abstract

Horizontal gene transfer has contributed to the global spread of the blaNDM-1 gene. Multiple studies have demonstrated plasmid transfer of blaNDM-1 between Gram-negative bacteria, primarily Enterobacteriaceae species, but conjugational transfer of natural blaNDM-1 plasmids from Enterobacteriaceae into Pseudomonas aeruginosa and Acinetobacter baumannii has not previously been shown. As P. aeruginosa and A. baumannii are both typically strong biofilm-formers, transfer of natural blaNDM-1 plasmids could potentially occur more readily in this environment. To determine whether natural blaNDM-1 plasmids could transfer to P. aeruginosa or A. baumannii in biofilms, three clinical and environmental Enterobacteriaceae strains carrying NDM-1-encoding plasmids of different incompatibility types were mated with E. coli J53, producing E. coli J53- blaNDM-1 transconjugants. Subsequently, dual-species biofilms were created using the E. coli J53 transconjugants as plasmid donors and either P. aeruginosa or A. baumannii as recipients. Biofilm transfer of NDM-encoding plasmids to P. aeruginosa and A. baumannii was successful from one and two E. coli J53- blaNDM-1 transconjugants, respectively. This demonstrates the potential for the spread of blaNDM-1, genes to P. aeruginosa and A. baumannii in clinical and environmental settings.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Uncontrolled Keywords:	NDM-1, biofilm, horizontal gene transfer, Pseudomonas aeruginosa, conjugation, Acinetobacter baumannii
Publisher:	Oxford University Press (OUP)
ISSN:	0378-1097
Date of First Compliant Deposit:	6 September 2017
Date of Acceptance:	24 February 2017
Last Modified:	25 Nov 2024 01:45
URI:	https://orca.cardiff.ac.uk/id/eprint/100913

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