Jurado, Jaime, De Navascues Melero, Joaquin  ORCID: https://orcid.org/0000-0002-5414-4056 and Gorfinkiel, Nicole
2016.
α-Catenin stabilises Cadherin-Catenin complexes and modulates actomyosin dynamics to allow pulsatile apical contraction.
Journal of Cell Science
, jcs.193268.
10.1242/jcs.193268
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Abstract
We have investigated how cell contractility and adhesion are functionally integrated during epithelial morphogenesis. To this end, we have analysed the role of α-Catenin, a key molecule linking E-Cadherin-based adhesion and the actomyosin cytoskeleton, during Drosophila embryonic dorsal closure, by studying a newly developed allelic series. We find that α-Catenin regulates pulsatile apical contraction in the amnioserosa, the main force-generating tissue driving closure of the embryonic epidermis. α-Catenin controls actomyosin dynamics by stabilising and promoting the formation of actomyosin foci, and also stabilises DE-Cadherin (Drosophila E-Cadherin, also known as Shotgun) at the cell membrane, suggesting that medioapical actomyosin contractility regulates junction stability. Furthermore, we uncover a genetic interaction between α-Catenin and Vinculin, and a tension-dependent recruitment of Vinculin to amniosersoa apical cell membranes, suggesting the existence of a mechano-sensitive module operating in this tissue.
| Item Type: | Article |
|---|---|
| Date Type: | Published Online |
| Status: | Published |
| Schools: | Schools > Biosciences Research Institutes & Centres > European Cancer Stem Cell Research Institute (ECSCRI) |
| Publisher: | The Company of Biologists Ltd |
| ISSN: | 0021-9533 |
| Date of First Compliant Deposit: | 26 June 2017 |
| Date of Acceptance: | 2 November 2016 |
| Last Modified: | 05 May 2023 03:45 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/101732 |
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