Gene expression analysis of rocket salad under pre-harvest and postharvest stresses: a transcriptomic resource for Diplotaxis tenuifolia

Cavaiuolo, Marina, Cocetta, Giacomo, Spadafora, Natasha D, Müller, Carsten Theodor ORCID: https://orcid.org/0000-0003-0455-7132, Rogers, Hilary Joan ORCID: https://orcid.org/0000-0003-3830-5857 and Ferrante, Antonio 2017. Gene expression analysis of rocket salad under pre-harvest and postharvest stresses: a transcriptomic resource for Diplotaxis tenuifolia. PLoS ONE 12 (5) , e0178119. 10.1371/journal.pone.0178119

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Abstract

Diplotaxis tenuifolia L. is of important economic value in the fresh-cut industry for its nutraceutical and sensorial properties. However, information on the molecular mechanisms conferring tolerance of harvested leaves to pre- and postharvest stresses during processing and shelf-life have never been investigated. Here, we provide the first transcriptomic resource of rocket by de novo RNA sequencing assembly, functional annotation and stress-induced expression analysis of 33874 transcripts. Transcriptomic changes in leaves subjected to commercially-relevant pre-harvest (salinity, heat and nitrogen starvation) and postharvest stresses (cold, dehydration, dark, wounding) known to affect quality and shelf-life were analysed 24h after stress treatment, a timing relevant to subsequent processing of salad leaves. Transcription factors and genes involved in plant growth regulator signaling, autophagy, senescence and glucosinolate metabolism were the most affected by the stresses. Hundreds of genes with unknown function but uniquely expressed under stress were identified, providing candidates to investigate stress responses in rocket. Dehydration and wounding had the greatest effect on the transcriptome and different stresses elicited changes in the expression of genes related to overlapping groups of hormones. These data will allow development of approaches targeted at improving stress tolerance, quality and shelf-life of rocket with direct applications in the fresh-cut industries.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Publisher:	Public Library of Science
ISSN:	1932-6203
Date of First Compliant Deposit:	4 July 2017
Date of Acceptance:	7 May 2017
Last Modified:	12 May 2023 08:11
URI:	https://orca.cardiff.ac.uk/id/eprint/102029

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