A dual-color far-red to near-infrared firefly luciferin analogue designed for multiparametric bioluminescence imaging

Jathoul, Amit P. ORCID: https://orcid.org/0000-0002-6767-7775, Grounds, Helen, Anderson, James C. and Pule, Martin A. 2014. A dual-color far-red to near-infrared firefly luciferin analogue designed for multiparametric bioluminescence imaging. Angewandte Chemie International Edition 53 (48) , pp. 13059-13063. 10.1002/anie.201405955

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Abstract

Red‐shifted bioluminescent emitters allow improved in vivo tissue penetration and signal quantification, and have led to the development of beetle luciferin analogues that elicit red‐shifted bioluminescence with firefly luciferase (Fluc). However, unlike natural luciferin, none have been shown to emit different colors with different luciferases. We have synthesized and tested the first dual‐color, far‐red to near‐infrared (nIR) emitting analogue of beetle luciferin, which, akin to natural luciferin, exhibits pH dependent fluorescence spectra and emits bioluminescence of different colors with different engineered Fluc enzymes. Our analogue produces different far‐red to nIR emission maxima up to λmax=706 nm with different Fluc mutants. This emission is the most red‐shifted bioluminescence reported without using a resonance energy transfer acceptor. This improvement should allow tissues to be more effectively probed using multiparametric deep‐tissue bioluminescence imaging.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Additional Information:	This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Publisher:	Wiley
ISSN:	1433-7851
Date of First Compliant Deposit:	24 March 2020
Last Modified:	04 May 2023 23:11
URI:	https://orca.cardiff.ac.uk/id/eprint/102051

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