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Topographical interrogation of the living cell surface reveals its role in rapid cell shape changes during phagocytosis and spreading

Jumaa, Maha A. Al, Dewitt, Sharon

and Hallett, Maurice B.

2017. Topographical interrogation of the living cell surface reveals its role in rapid cell shape changes during phagocytosis and spreading. Scientific Reports 7 , -. 10.1038/s41598-017-09761-6

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Official URL: http://dx.doi.org/10.1038/s41598-017-09761-6

Abstract

Dramatic and rapid changes in cell shape are perhaps best exemplified by phagocytes, such as neutrophils. These cells complete the processes of spreading onto surfaces, and phagocytosis within 100 s of stimulation. Although these cell shape changes are accompanied by an apparent large increase in cell surface area, the nature of the membrane “reservoir” for the additional area is unclear. One proposal is that the wrinkled cell surface topography (which forms micro-ridges on the neutrophil surface) provides the resource for neutrophils to expand their available surface area. However, it has been problematic to test this proposal in living cells because these surface structures are sub-light microscopic. In this paper, we report the development of a novel approach, a variant of FRAP (fluorescent recovery after photo-bleaching) modified to interrogate the diffusion path-lengths of membrane associated molecules. This approach provides clear evidence that the cell surface topography changes dramatically during neutrophil shape change (both locally and globally) and can be triggered by elevating cytosolic Ca2+.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry
Additional Information:	Note: two movies are available from the links in the Supplementary Appendix tab on the article web page. https://www.nature.com/articles/s41598-017-09761-6#Sec17
Publisher:	Nature Publishing Group
ISSN:	2045-2322
Date of First Compliant Deposit:	31 August 2017
Date of Acceptance:	28 July 2017
Last Modified:	06 Nov 2024 17:45
URI:	https://orca.cardiff.ac.uk/id/eprint/104069