Yusop, Norhayati, Battersby, Paul  ORCID: https://orcid.org/0000-0001-8562-9761, Alraies, Amr ORCID: https://orcid.org/0000-0003-1977-3487, Sloan, Alastair J ORCID: https://orcid.org/0000-0002-1791-0903, Moseley, Ryan ORCID: https://orcid.org/0000-0002-2812-6735 and Waddington, Rachel J.
2018.
Isolation and characterisation of mesenchymal stem cells from rat bone marrow and the endosteal niche: A comparative study.
Stem Cells International
, 6869128.
10.1155/2018/6869128
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Abstract
Within bone, mesenchymal stromal cells (MSCs) exist within the bone marrow stroma (BM-MSC) and the endosteal niche, as cells lining compact bone (CB-MSCs). This study isolated and characterised heterogeneous MSC populations from each niche and subsequently investigated the effects of extensive cell expansion, analysing population doublings (PDs)/cellular senescence, colony-forming efficiencies (CFEs), MSC cell marker expression, and osteogenic/adipogenic differentiation. CB-MSCs and BM-MSCs demonstrated similar morphologies and PDs, reaching 100 PDs. Both populations exhibited consistent telomere lengths (12–17 kb), minimal senescence, and positive telomerase expression. CB-MSCs (PD15) had significantly lower CFEs than PD50. CB-MSCs and BM-MSCs both expressed MSC (CD73/CD90/CD105); embryonic (Nanog) and osteogenic markers (Runx2, osteocalcin) but no hematopoietic markers (CD45). CB-MSCs (PD15) strongly expressed Oct4 and p16INK4A. At early PDs, CB-MSCs possessed a strong osteogenic potency and low potency for adipogenesis, whilst BM-MSCs possessed greater overall bipotentiality for osteogenesis and adipogenesis. At PD50, CB-MSCs demonstrated reduced potency for both osteogenesis and adipogenesis, compared to BM-MSCs at equivalent PDs. This study demonstrates similarities in proliferative and mesenchymal cell characteristics between CB-MSCs and BM-MSCs, but contrasting multipotentiality. Such findings support further comparisons of human CB-MSCs and BM-MSCs, facilitating selection of optimal MSC populations for regenerative medicine purposes.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | Schools > Dentistry |
| Publisher: | Hindawi Publishing Corporation |
| ISSN: | 1687-9678 |
| Related URLs: | |
| Date of First Compliant Deposit: | 23 November 2017 |
| Date of Acceptance: | 5 November 2017 |
| Last Modified: | 16 Nov 2024 00:45 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/107002 |
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