Isolation and characterisation of mesenchymal stem cells from rat bone marrow and the endosteal niche: A comparative study

Yusop, Norhayati, Battersby, Paul ORCID: https://orcid.org/0000-0001-8562-9761, Alraies, Amr ORCID: https://orcid.org/0000-0003-1977-3487, Sloan, Alastair J ORCID: https://orcid.org/0000-0002-1791-0903, Moseley, Ryan ORCID: https://orcid.org/0000-0002-2812-6735 and Waddington, Rachel J. 2018. Isolation and characterisation of mesenchymal stem cells from rat bone marrow and the endosteal niche: A comparative study. Stem Cells International , 6869128. 10.1155/2018/6869128

Preview

PDF - Accepted Post-Print Version Available under License Creative Commons Attribution. Download (1MB) | Preview

Abstract

Within bone, mesenchymal stromal cells (MSCs) exist within the bone marrow stroma (BM-MSC) and the endosteal niche, as cells lining compact bone (CB-MSCs). This study isolated and characterised heterogeneous MSC populations from each niche and subsequently investigated the effects of extensive cell expansion, analysing population doublings (PDs)/cellular senescence, colony-forming efficiencies (CFEs), MSC cell marker expression, and osteogenic/adipogenic differentiation. CB-MSCs and BM-MSCs demonstrated similar morphologies and PDs, reaching 100 PDs. Both populations exhibited consistent telomere lengths (12–17 kb), minimal senescence, and positive telomerase expression. CB-MSCs (PD15) had significantly lower CFEs than PD50. CB-MSCs and BM-MSCs both expressed MSC (CD73/CD90/CD105); embryonic (Nanog) and osteogenic markers (Runx2, osteocalcin) but no hematopoietic markers (CD45). CB-MSCs (PD15) strongly expressed Oct4 and p16INK4A. At early PDs, CB-MSCs possessed a strong osteogenic potency and low potency for adipogenesis, whilst BM-MSCs possessed greater overall bipotentiality for osteogenesis and adipogenesis. At PD50, CB-MSCs demonstrated reduced potency for both osteogenesis and adipogenesis, compared to BM-MSCs at equivalent PDs. This study demonstrates similarities in proliferative and mesenchymal cell characteristics between CB-MSCs and BM-MSCs, but contrasting multipotentiality. Such findings support further comparisons of human CB-MSCs and BM-MSCs, facilitating selection of optimal MSC populations for regenerative medicine purposes.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry
Publisher:	Hindawi Publishing Corporation
ISSN:	1687-9678
Related URLs:	https://www.hindawi.com/journals/sci/aip...
Date of First Compliant Deposit:	23 November 2017
Date of Acceptance:	5 November 2017
Last Modified:	16 Nov 2023 07:17
URI:	https://orca.cardiff.ac.uk/id/eprint/107002

Citation Data

Cited 42 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item