Control analysis of lipid biosynthesis in tissue cultures from oil crops shows that flux control is shared between fatty acid synthesis and lipid assembly

Ramli, Umi S., Baker, Darren S., Quant, Patti A. and Harwood, John ORCID: https://orcid.org/0000-0003-2377-2612 2002. Control analysis of lipid biosynthesis in tissue cultures from oil crops shows that flux control is shared between fatty acid synthesis and lipid assembly. Biochemical Journal 364 , pp. 393-401. 10.1042/BJ20010203

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Abstract

Top-Down (Metabolic) Control Analysis (TDCA) was used to examine, quantitatively, lipid biosynthesis in tissue cultures from two commercially important oil crops, olive (Olea europaea L.) and oil palm (Elaeis guineensis Jacq.). A conceptually simplified system was defined comprising two blocks of reactions: fatty acid synthesis (Block A) and lipid assembly (Block B), which produced and consumed, respectively, a common and unique system intermediate, cytosolic acyl-CoA. We manipulated the steady-state levels of the system intermediate by adding exogenous oleic acid and, using two independent assays, measured the effect of the addition on the system fluxes (JA and JB). These were the rate of incorporation of radioactivity: (i) through Block A from [1-14C]acetate into fatty acids and (ii) via Block B from [U-14C]glycerol into complex lipids respectively. The data showed that fatty acid formation (Block A) exerted higher control than lipid assembly (Block B) in both tissues with the following group flux control coefficients (C):(i) Oil palm: *CJTL/BikA=0.64

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Subjects:	Q Science > QH Natural history > QH301 Biology
Uncontrolled Keywords:	oil palm (Elaeis guineensis Jacq.) ; olive (Olea europaea L.) ; Top-Down Control Analysis ; triacylglycerol production
Publisher:	Portland Press
ISSN:	0264-6021
Last Modified:	17 Oct 2022 08:48
URI:	https://orca.cardiff.ac.uk/id/eprint/1099

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