Role of MAG1 proteins in invasiveness of human colorectal cancer cells

Khanzada, Zubair 2018. Role of MAG1 proteins in invasiveness of human colorectal cancer cells. MD Thesis, Cardiff University. Item availability restricted.

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Abstract

Introduction: MAGI -1 plays an essential role in cancer metastasis. We aimed to study the expression of MAGI -1, -2 and -3 in colorectal cancer tissue with specific focus on the role of MAGI -1 in colorectal cancer metastasis, through its function in the maintenance of cell-to-cell tight junctions. Materials and Methods: MAGI -1, -2 and -3, expression was determined in colorectal cancer tissue by using Q-PCR and RT-PCR. MAGI -1 knockdowns were created in cancer cell lines by electroporation method and were confirmed on RT- PCR, morphology and immunofluorescence. The impact of aberrantly expressed MAGI -1 on adhesion, invasion and migration of colorectal cancer tissue was studied by using trans-epithelial resistance (TER) and electrical cell impedance (ECIS) assays. Results: Q-PCR was consistent with the reduced expression of MAGI-1, -2 and -3 in colorectal cancer tissue. Survival curves showed reduced survival in cohort with reduced MAGI expression. MAGI -2 lacked expression on RT-PCR. MAGI -1 KDs showed decrease trans-epithelial resistance in the KDs. Reduced electrical resistance across epithelial cell lines in MAGI -1 KDs and also reduced adhesion and increase in migratory function was observed in MAGI -1 KDs on Trans-epithelial resistance (TER) and electronic cell impedance sensing (ECIS) experiments respectively. Conclusion: Our experimental work suggests that MAGI-1 plays a vital role in maintaining the barrier function of tight-junctions in colorectal cancer tissue, which when knocked down, has a significant implication in colorectal cancer metastasis.

Item Type:	Thesis (MD)
Status:	Unpublished
Schools:	Medicine
Date of First Compliant Deposit:	29 May 2018
Last Modified:	13 Apr 2021 13:56
URI:	https://orca.cardiff.ac.uk/id/eprint/111760

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