Watson, Peter Duncan ORCID: https://orcid.org/0000-0003-0250-7852, Forster, Rebecca, Palmer, Krysten J., Pepperkok, Rainer and Stephens, David J. 2004. Coupling of ER exit to microtubules through direct interaction of COPII with dynactin. Nature Cell Biology 7 , pp. 48-55. 10.1038/ncb1206 |
Abstract
Transport of proteins from the endoplasmic reticulum (ER) to the Golgi is mediated by the sequential action of two coat complexes: COPII concentrates cargo for secretion at ER export sites, then COPI is subsequently recruited to nascent carriers and retrieves recycling proteins back to the ER. These carriers then move towards the Golgi along microtubules, driven by the dynein/dynactin complexes. Here we show that the Sec23p component of the COPII complex directly interacts with the dynactin complex through the carboxy-terminal cargo-binding domain of p150Glued. Functional assays, including measurements of the rate of recycling of COPII on the ER membrane and quantitative analyses of secretion, indicate that this interaction underlies functional coupling of ER export to microtubules. Together, our data suggest a mechanism by which membranes of the early secretory pathway can be linked to motors and microtubules for subsequent organization and movement to the Golgi apparatus.
Item Type: | Article |
---|---|
Date Type: | Publication |
Status: | Published |
Schools: | Biosciences |
Subjects: | Q Science > QH Natural history > QH301 Biology |
ISSN: | 1465-7392 |
Last Modified: | 17 Oct 2022 08:48 |
URI: | https://orca.cardiff.ac.uk/id/eprint/1122 |
Citation Data
Cited 138 times in Scopus. View in Scopus. Powered By Scopus® Data
Actions (repository staff only)
Edit Item |